Biology Is Love

 Crabtree effect: The name is given by an English Biochemist Herbert Grace Crabtree.  This effect is referred to as the production of ethanol in aerobic conditions rather than biomass by yeast cells at high external glucose concentrations through the TCA cycle.  This is basically the decrease in the rate of electron transport due to the mass action of the elevated phosphorylation ratio.  Warburg effect: This effect is referred to as an increase in the rate of glucose uptake and the production of lactate in aerobic conditions. The discoverer of this effect,  Warburg hypothesized that this effect is mainly due to the dysfunctional mitochondria, observed in the tumor cells, predominantly found in the cancer developmental cells.  The Crabtree-Warburg effect interprets that the avoidance of a decrease in ATP is observed in those conditions where higher glucose uptake is found than the downstream processing capability of the second phase of the glycolysis. 

  MTT Assay Principle : The MTT assay provides a readout of cell viability & growth that works by measuring cell metabolic activity. The yellow tetrazolium MTT (3-(4, 5-dimethyl thiazolyl-2)-2, 5-diphenyltetrazolium bromide) is reduced by the metabolically active cells, by the action of dehydrogenase enzymes, to produce reducing equivalents such as NADH and NADPH. The resulting intracellular purple formazan can be solubilized and quantified by spectrophotometric means at 570nm. MTT is sensitive to light. This assay should be performed in the dark. The MTT assay measures,  cellular metabolic activity as an indicator of cell viability, proliferation and cytotoxicity.

The Basic steps of the cell-reviving process   1)   Cryopreserved Cells [with DMSO (Dimethyl sulfoxide)] were collected from the -80°C. 2)  Then the cells were thawed rapidly in the water bath at 37 °C for 30-45 sec to protect the cells from DMSO. 3)  The cells were transferred into a 15 ml falcon which already contains 6 ml of media [DMEM (Dulbecco’s Modified Eagle Medium) + 10% FBS (Foetal Bovine Serum) + 1X penicillin-streptomycin solution]. 4)  The centrifugation was performed at 1100 rpm for 5 minutes. 5)  The Supernatant was discarded in the beaker containing a small amount of bleach and water.  The pellet was collected. 6) T he pellet was washed with media to remove the trace of DMSO. 7)  The cells are resuspended in 1 ml of media.   8)  The cells were transferred to the T-25 flask which already contains 4 ml of media.  9)  Finally, the cells were observed under the microscope and kept in the incubator at 37°...

  Cell biology is the study of cell structure and function, and it revolves around the concept that the cell is the fundamental unit of life.If you want to Know about biology then at first you have to know about cell.  So here is the list of top 5 book on Cell biology for you. 1. Molecular biology of THE CELL   by  Bruce Alberts  &  Alexander Johnson  &  Julian Lewis  &  David Morgan  &  Martin Raff  &  Keith Roberts  &  Peter Walter This book is called father of cell biology. It is filled with so much of information about cell. Click here to buy 2.  The Cell. A Molecular Approach by Cooper CLICK HERE TO DOWNLOAD PDF Click on the Image to buy 3.  Molecular Cell Biology by Lodish CLICK HERE TO DOWNLOAD Buy Now 4.  Lewin's Cells Buy now 5.    Becker’s World of the Cell (9th edition) Click here to Buy CONTACT US FOR FREE PDF OF THESE BOOK.

 BCL2 PROTEINS REGULATES INTRINSIC PATHWAY OF APOPTOSIS                                                                                   -EISHIKA DAS In Intrinsic apoptotic pathway, mitochondrion plays a central role by releasing cytochrome C. The defining event during intrinsic apoptotic pathway Is mitochondrial outer membrane permeabilization or MOMP . Following MOMP, cytochrome c, are released into the cytosol. Once in the cytoplasm, cytochrome c binds to adaptor protein APAF1 . This interaction leads to hydrolysis of the bound dATP to dADP and a change in the conformation of APAF-1.  APAF-1 assembles into a heptameric complex, the apoptosome . The APAF-1 proteins in the apoptosome then recruit initiator procaspase-9. The incorporation of procaspase-9 into the apoptosome ...