Skip to main content

Principle of MTT assay

 

MTT Assay

Principle:

The MTT assay provides a readout of cell viability & growth that works by measuring cell metabolic activity. The yellow tetrazolium MTT (3-(4, 5-dimethyl thiazolyl-2)-2, 5-diphenyltetrazolium bromide) is reduced by the metabolically active cells, by the action of dehydrogenase enzymes, to produce reducing equivalents such as NADH and NADPH. The resulting intracellular purple formazan can be solubilized and quantified by spectrophotometric means at 570nm.

MTT is sensitive to light. This assay should be performed in the dark.

The MTT assay measures, cellular metabolic activity as an indicator of cell viability, proliferation and cytotoxicity.




Labels:

Comments

Post a Comment

please share it with your friends and students

Popular posts from this blog

TOP 5 BOOKS ON HUMAN PHYSIOLOGY

Post a Comment
Read more

COVID 19 - A brief details about COVID-19 , HISTORY, STRUCTURE, MODE OF TRANSMISSION, MODE OF ACTION and SYMPTOMS

 COVID-19 - Eishika Das,  -Soumyaprabha Ganguly HISTORY:  Coronavirus disease 2019  ( COVID-19 ) is an  infectious disease  caused by  severe acute respiratory syndrome coronavirus 2  (SARS-CoV-2) The disease was first identified in 2019 in  Wuhan , the capital of  Hubei , China, and has since spread globally, resulting in the  2019–20 coronavirus pandemic . According to the World Health Organization (WHO), viral diseases continue to emerge and represent a serious issue to public health. In the last twenty years, several viral epidemics such as the severe acute respiratory syndrome coronavirus (SARS-CoV) in 2002 to 2003 beginning in China and involving two dozen countries with approximately 8000 cases and 800 deaths , and H1N1 influenza in 2009, have been recorded. Most recently, the Middle East respiratory syndrome coronavirus (MERS-CoV) was first identified in Saudi Arabia in 2012 approximately 2,500 cases and 800...
Post a Comment
Read more

FREE RESEARCH PAPER FOR EVERYONE AROUND THE WORLD

  ANY KIND OF SCIENCE RESEARCH PAPER FOR FREE!!!!!!!! YES, YOU CAN GET IT FOR FREE Sci-Hub yes, Sci-Hub --The first website in the world to provide mass and public access to millions of research paper for free . yes it is a pirate website . They get copyright issues many times but they continue their work.  their moto is- "knowledge to all" they have not any particular url or website link.  HOW TO DOWNLOAD YOUR PAPER OF INTEREST: Go to  https://scholar.google.co.in Search for your research paper of interest and go to respective link. Copy the URL of that page, or copy the PMID/DOI of the paper Now go to any search engine (i.e. google , bing, etc)and search for Sci-Hub go to the website   https://scihub.wikicn.top/ Paste the link and download. If you are capable, you can donate some amount to  Sci-hub. THANK YOU
Post a Comment
Read more