Biology Is Love

The Basic steps of the cell-reviving process   1)   Cryopreserved Cells [with DMSO (Dimethyl sulfoxide)] were collected from the -80°C. 2)  Then the cells were thawed rapidly in the water bath at 37 °C for 30-45 sec to protect the cells from DMSO. 3)  The cells were transferred into a 15 ml falcon which already contains 6 ml of media [DMEM (Dulbecco’s Modified Eagle Medium) + 10% FBS (Foetal Bovine Serum) + 1X penicillin-streptomycin solution]. 4)  The centrifugation was performed at 1100 rpm for 5 minutes. 5)  The Supernatant was discarded in the beaker containing a small amount of bleach and water.  The pellet was collected. 6) T he pellet was washed with media to remove the trace of DMSO. 7)  The cells are resuspended in 1 ml of media.   8)  The cells were transferred to the T-25 flask which already contains 4 ml of media.  9)  Finally, the cells were observed under the microscope and kept in the incubator at 37°...

  UNIT 1 pH concept amino acid properties Ramachandran plot Km and Vmax Inhibition Kinetics Enzymatic degradation of Protein Structure and function of DNA and RNA Super coiling Cofactor and Coenzyme Protein Structure (alpha-helix and beta-sheet and tern) UNIT 2 Chromatin and Nucleosome Function of all organelle Transport across membrane Lipid Raft Protein trafficking Endocytosis and phagocytosis Cell division (Check Points) Membrane Cell junction Rb and P53 gene in cancer CDK and Cyclin UNIT 3 Structure and Function of DNA Polymerase in Prokaryotic and Eukaryotic cell Structure and Function of RNA Polymerase in Prokaryotic and Eukaryotic cell Replication Transcription Translation UNIT 4 Entry of virus in different host Pathogen-mediated disease GPCR, RTK, cell signaling pathway Cell communication, adhesion, and junction Cancer genes

Fertilization In Sea Urchin - Malay Nayak 1.        What is sea urchin? ·          Spiny water animal ·          Phylum ECHINODERMATA ·          Roughly spherical sea animal Sea Urchin   2. Why Sea Urchin ? The cell-surface events that take place during fertilization have been studied most extensively in sea urchins, members of the phylum Echinodermata . ·    Sea urchin gametes are easy to collect, ·    fertilization occurs outside the animal body. ·    As a result, researchers can observe fertilization and subsequent development simply by combining eggs and sperm in seawater in the laboratory.