CSIR NET LIFE SCIENCE IMPORTANT TOPICS CHAPTERWISE WITH TOP RECOMMENDED BOOKS

UNIT 1

pH concept
amino acid properties
Ramachandran plot
Km and Vmax
Inhibition Kinetics
Enzymatic degradation of Protein
Structure and function of DNA and RNA
Super coiling
Cofactor and Coenzyme
Protein Structure (alpha-helix and beta-sheet and tern)

UNIT 2

Chromatin and Nucleosome
Function of all organelle
Transport across membrane
Lipid Raft
Protein trafficking
Endocytosis and phagocytosis
Cell division (Check Points)
Membrane
Cell junction
Rb and P53 gene in cancer
CDK and Cyclin

UNIT 3

Structure and Function of DNA Polymerase in Prokaryotic and Eukaryotic cell
Structure and Function of RNA Polymerase in Prokaryotic and Eukaryotic cell
Replication
Transcription
Translation

UNIT 4

Entry of virus in different host
Pathogen-mediated disease
GPCR, RTK, cell signaling pathway
Cell communication, adhesion, and junction
Cancer genes

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Are Apple Seeds Poisonous? The Truth Behind Cyanide Content

Cyanide in Apple!! 🍏 Apple 🍎seeds are often regarded as a healthy snack that is packed with numerous nutritional benefits. However, there is a concern that apple seeds contain cyanide, a toxic substance that can cause harm to the human body. In this article, we will discuss the truth behind apple seeds containing cyanide, how much cyanide is present, and whether eating apple seeds is safe or not. The truth behind apple seeds containing cyanide Apple seeds contain a small amount of cyanide, which is a poisonous chemical compound that can be harmful to the body if ingested in large amounts. The cyanide is present in the form of amygdalin, which is a glycoside that is found in the seeds of various fruits, including apples. When apple seeds are crushed or chewed, amygdalin is released and can be converted into hydrogen cyanide, a poisonous gas. How much cyanide is present in apple seeds? The amount of cyanide present in apple seeds varies depending on the variety of apple and the ...

Principle of MTT assay

MTT Assay Principle : The MTT assay provides a readout of cell viability & growth that works by measuring cell metabolic activity. The yellow tetrazolium MTT (3-(4, 5-dimethyl thiazolyl-2)-2, 5-diphenyltetrazolium bromide) is reduced by the metabolically active cells, by the action of dehydrogenase enzymes, to produce reducing equivalents such as NADH and NADPH. The resulting intracellular purple formazan can be solubilized and quantified by spectrophotometric means at 570nm. MTT is sensitive to light. This assay should be performed in the dark. The MTT assay measures, cellular metabolic activity as an indicator of cell viability, proliferation and cytotoxicity.

INTRODUCTION TO RECOMBINANT DNA TECHNOLOGY(RDT) WITH ITS STEP AND APPLICATION

INTRODUCTION TO RECOMBINANT DNA TECHNOLOGY(RDT) - Devlina Sarkar v What is Recombinant DNA technology ? RDT is a series of procedures that is used to join together (recombine) DNA segments from different organisms and inserting it into a host organism to produce new genetic combinations. v What is called Recombinant DNA ? Recombinant DNA (rDNA) is m olecules of DNA from two different species that are inserted into a host organism to produce new genetic combinations that are of value to science, medicine, agriculture, and industry. Recombinant DNA in a living organism was first achieved in 1973 by Herbert Boyer , of the University of California at San Francisco, and Stanley Cohen , at Stanford University , who used E. coli restriction enzymes to insert foreign DNA into plasmids. v What is the basic use of RDT? Using RDT we can get a huge no of copies/clones of a particular gene or DNA(cloning) by usi...

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