Skip to main content

CSIR NET LIFE SCIENCE IMPORTANT TOPICS CHAPTERWISE WITH TOP RECOMMENDED BOOKS

 UNIT 1

  • pH concept
  • amino acid properties
  • Ramachandran plot
  • Km and Vmax
  • Inhibition Kinetics
  • Enzymatic degradation of Protein
  • Structure and function of DNA and RNA
  • Super coiling
  • Cofactor and Coenzyme
  • Protein Structure (alpha-helix and beta-sheet and tern)
UNIT 2

  • Chromatin and Nucleosome
  • Function of all organelle
  • Transport across membrane
  • Lipid Raft
  • Protein trafficking
  • Endocytosis and phagocytosis
  • Cell division (Check Points)
  • Membrane
  • Cell junction
  • Rb and P53 gene in cancer
  • CDK and Cyclin
UNIT 3

  • Structure and Function of DNA Polymerase in Prokaryotic and Eukaryotic cell
  • Structure and Function of RNA Polymerase in Prokaryotic and Eukaryotic cell
  • Replication
  • Transcription
  • Translation
UNIT 4
  • Entry of virus in different host
  • Pathogen-mediated disease
  • GPCR, RTK, cell signaling pathway
  • Cell communication, adhesion, and junction
  • Cancer genes

Labels:

Comments

Post a Comment

please share it with your friends and students

Popular posts from this blog

TOP 5 BOOKS ON HUMAN PHYSIOLOGY

Post a Comment
Read more

INTRODUCTION TO RECOMBINANT DNA TECHNOLOGY(RDT) WITH ITS STEP AND APPLICATION

  INTRODUCTION TO RECOMBINANT DNA TECHNOLOGY(RDT) - Devlina Sarkar v What is Recombinant DNA technology ? RDT is a series of procedures that is used to join together (recombine) DNA segments from different organisms and inserting it into a host organism to produce new genetic combinations. v What is called Recombinant DNA ? Recombinant DNA (rDNA) is   m olecules  of  DNA  from two different species that are inserted into a host organism to produce new genetic combinations that are of value to science, medicine, agriculture, and industry. Recombinant DNA in a living organism was first achieved in 1973 by  Herbert Boyer , of the University of California at San Francisco, and  Stanley Cohen , at Stanford University , who used   E. coli  restriction enzymes to insert foreign DNA into plasmids. v What is the basic use of RDT? Using RDT we can get a huge no of copies/clones of a particular gene or DNA(cloning) by usi...
Post a Comment
Read more

Principle of MTT assay

  MTT Assay Principle : The MTT assay provides a readout of cell viability & growth that works by measuring cell metabolic activity. The yellow tetrazolium MTT (3-(4, 5-dimethyl thiazolyl-2)-2, 5-diphenyltetrazolium bromide) is reduced by the metabolically active cells, by the action of dehydrogenase enzymes, to produce reducing equivalents such as NADH and NADPH. The resulting intracellular purple formazan can be solubilized and quantified by spectrophotometric means at 570nm. MTT is sensitive to light. This assay should be performed in the dark. The MTT assay measures,  cellular metabolic activity as an indicator of cell viability, proliferation and cytotoxicity.
Post a Comment
Read more