Biology Is Love

  MTT Assay Principle : The MTT assay provides a readout of cell viability & growth that works by measuring cell metabolic activity. The yellow tetrazolium MTT (3-(4, 5-dimethyl thiazolyl-2)-2, 5-diphenyltetrazolium bromide) is reduced by the metabolically active cells, by the action of dehydrogenase enzymes, to produce reducing equivalents such as NADH and NADPH. The resulting intracellular purple formazan can be solubilized and quantified by spectrophotometric means at 570nm. MTT is sensitive to light. This assay should be performed in the dark. The MTT assay measures,  cellular metabolic activity as an indicator of cell viability, proliferation and cytotoxicity.

The Basic steps of the cell-reviving process   1)   Cryopreserved Cells [with DMSO (Dimethyl sulfoxide)] were collected from the -80°C. 2)  Then the cells were thawed rapidly in the water bath at 37 °C for 30-45 sec to protect the cells from DMSO. 3)  The cells were transferred into a 15 ml falcon which already contains 6 ml of media [DMEM (Dulbecco’s Modified Eagle Medium) + 10% FBS (Foetal Bovine Serum) + 1X penicillin-streptomycin solution]. 4)  The centrifugation was performed at 1100 rpm for 5 minutes. 5)  The Supernatant was discarded in the beaker containing a small amount of bleach and water.  The pellet was collected. 6) T he pellet was washed with media to remove the trace of DMSO. 7)  The cells are resuspended in 1 ml of media.   8)  The cells were transferred to the T-25 flask which already contains 4 ml of media.  9)  Finally, the cells were observed under the microscope and kept in the incubator at 37°...

  UNIT 1 pH concept amino acid properties Ramachandran plot Km and Vmax Inhibition Kinetics Enzymatic degradation of Protein Structure and function of DNA and RNA Super coiling Cofactor and Coenzyme Protein Structure (alpha-helix and beta-sheet and tern) UNIT 2 Chromatin and Nucleosome Function of all organelle Transport across membrane Lipid Raft Protein trafficking Endocytosis and phagocytosis Cell division (Check Points) Membrane Cell junction Rb and P53 gene in cancer CDK and Cyclin UNIT 3 Structure and Function of DNA Polymerase in Prokaryotic and Eukaryotic cell Structure and Function of RNA Polymerase in Prokaryotic and Eukaryotic cell Replication Transcription Translation UNIT 4 Entry of virus in different host Pathogen-mediated disease GPCR, RTK, cell signaling pathway Cell communication, adhesion, and junction Cancer genes

  Keto diet: How does this diet work: The establishment of the keto diet is if glucose (the simplest form of carbohydrate as well as the main source of energy in every animal) is deprived, which is gained by consuming carbs. The ketones are a substitution of glucose, which is synthesized from stored fat. Hence, the name is ketogenic. The neurocytes (The brain cells) need glucose approximately 120 grams per day because these cells cannot store glucose. When a person is fasting or consumes very little carbohydrates, at first the body pulls glucose from the liver and muscle glycogen. Glycogen breaks down from the liver and muscle into glucose through glycogenolysis by the influence of the hormone glucagon. During this time the level of insulin gets decreased in the blood. The body starts utilizing fat as its primary fuel. When formed ketone bodies begin to accumulate in the organs as well as in the blood, the condition is called ketosis. Mild ketosis is usually experienced by e...