General notes for life science competitive exams (mainly SET)

The following notes are the handwritten notes for Life science competitive exams (mainly SET). It is helpful for UGC NET too.

If you face any kind of problems please let us know in the comment section.

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TOP 5 BOOKS ON HUMAN PHYSIOLOGY

INTRODUCTION TO RECOMBINANT DNA TECHNOLOGY(RDT) WITH ITS STEP AND APPLICATION

INTRODUCTION TO RECOMBINANT DNA TECHNOLOGY(RDT) - Devlina Sarkar v What is Recombinant DNA technology ? RDT is a series of procedures that is used to join together (recombine) DNA segments from different organisms and inserting it into a host organism to produce new genetic combinations. v What is called Recombinant DNA ? Recombinant DNA (rDNA) is m olecules of DNA from two different species that are inserted into a host organism to produce new genetic combinations that are of value to science, medicine, agriculture, and industry. Recombinant DNA in a living organism was first achieved in 1973 by Herbert Boyer , of the University of California at San Francisco, and Stanley Cohen , at Stanford University , who used E. coli restriction enzymes to insert foreign DNA into plasmids. v What is the basic use of RDT? Using RDT we can get a huge no of copies/clones of a particular gene or DNA(cloning) by usi...

Principle of MTT assay

MTT Assay Principle : The MTT assay provides a readout of cell viability & growth that works by measuring cell metabolic activity. The yellow tetrazolium MTT (3-(4, 5-dimethyl thiazolyl-2)-2, 5-diphenyltetrazolium bromide) is reduced by the metabolically active cells, by the action of dehydrogenase enzymes, to produce reducing equivalents such as NADH and NADPH. The resulting intracellular purple formazan can be solubilized and quantified by spectrophotometric means at 570nm. MTT is sensitive to light. This assay should be performed in the dark. The MTT assay measures, cellular metabolic activity as an indicator of cell viability, proliferation and cytotoxicity.

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