Skip to main content

Comments

Post a Comment

please share it with your friends and students

Popular posts from this blog

Why oxytocin and vasopressin called as 'neuro hormone'?

  Why Oxytocin and Vasopressin called as 'neuro hormone'?      -souti das                            The posterior pitutary secretes two hormone oxytocin and vasopressin(ADH).The hormones are synthesized within the cell bodies of large magnocellular neurons lying in the supraoptic and paraventricular nuclei (PVN) of the hypothalamus.                            The posterior pituitary hormones are transported in association with specific proteins, called neurophysins , along the axons of the neurons to end in those nerve terminals which lie within the posterior lobe. Prior to secretion, these hormones are stored in secretory granules either in the nerve terminals or in the varicosites called herring bodies . The hormones are secreted in response to nerve impulses originating in the supraoptic and PVN and enter the capill...
Post a Comment
Read more

ANAMMOX -- Anaerobic ammonium oxidation

  ANAMMOX - Nandini Das Anammox is an abbreviation for anaerobic ammonium oxidation , is a globally important microbial process of the nitrogen cycle.
Post a Comment
Read more

The Basic steps of the cell-reviving process

The Basic steps of the cell-reviving process   1)   Cryopreserved Cells [with DMSO (Dimethyl sulfoxide)] were collected from the -80°C. 2)  Then the cells were thawed rapidly in the water bath at 37 °C for 30-45 sec to protect the cells from DMSO. 3)  The cells were transferred into a 15 ml falcon which already contains 6 ml of media [DMEM (Dulbecco’s Modified Eagle Medium) + 10% FBS (Foetal Bovine Serum) + 1X penicillin-streptomycin solution]. 4)  The centrifugation was performed at 1100 rpm for 5 minutes. 5)  The Supernatant was discarded in the beaker containing a small amount of bleach and water.  The pellet was collected. 6) T he pellet was washed with media to remove the trace of DMSO. 7)  The cells are resuspended in 1 ml of media.   8)  The cells were transferred to the T-25 flask which already contains 4 ml of media.  9)  Finally, the cells were observed under the microscope and kept in the incubator at 37°...
Post a Comment
Read more